quantitative PCR Insights

What is quantitative PCR?

Quantitative PCR (qPCR), also known as real-time PCR, is a laboratory technique used to amplify and quantify a targeted DNA molecule. It enables both detection and quantification (measuring) of a specific DNA sequence in a sample. It is commonly used in molecular biology, microbiology, and diagnostics for applications such as gene expression analysis, pathogen detection, and cancer research.

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What other technologies are related to quantitative PCR?

quantitative PCR Competitor Technologies

PCR

PCR amplifies DNA, similar to qPCR, making it a direct competitor.

mentioned alongside quantitative PCR in 1% (571) of relevant job posts

RT-PCR

RT-PCR amplifies RNA, which can be an alternative to qPCR.

mentioned alongside quantitative PCR in 2% (151) of relevant job posts

digital PCR

Digital PCR is another method of nucleic acid quantification, and therefore it is a competitor.

mentioned alongside quantitative PCR in 5% (54) of relevant job posts

next-generation sequencing

Next-generation sequencing provides comprehensive sequence information and gene expression analysis, which can be a competitor to qPCR for gene expression studies.

mentioned alongside quantitative PCR in 1% (155) of relevant job posts

RNA-seq

RNA-seq is a method for quantifying RNA transcripts and can be used to determine relative RNA abundance and has the same uses as qPCR. RNA-seq provides a more comprehensive, genome-wide, view of transcription.

mentioned alongside quantitative PCR in 2% (83) of relevant job posts

real-time PCR

Real-time PCR is another name for qPCR and serves the same function.

mentioned alongside quantitative PCR in 2% (68) of relevant job posts