Next-generation sequencing (NGS) encompasses a variety of high-throughput sequencing technologies that have revolutionized genomic research. Unlike Sanger sequencing, which sequences a single DNA fragment at a time, NGS technologies sequence millions or billions of DNA fragments in parallel, massively increasing throughput and speed. NGS is commonly used for whole-genome sequencing, exome sequencing, targeted sequencing, RNA sequencing (RNA-Seq), and metagenomics, enabling researchers to study genetic variation, gene expression, and other biological processes at an unprecedented scale.
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