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Sanger sequencing

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**Sanger sequencing**

What is Sanger sequencing?

Sanger sequencing, also known as chain-termination sequencing, is a method for determining the nucleotide sequence of DNA. It involves synthesizing a new DNA strand complementary to the template strand, but incorporating modified nucleotides (dideoxynucleotides) that terminate DNA synthesis. These terminated fragments are then separated by size, allowing the sequence to be read. It is commonly used for confirming DNA sequences, sequencing individual genes or small genomes, and for mutation detection.

Find 498 organizations using Sanger sequencing on Sumble →

What other technologies are related to Sanger sequencing?

Sanger sequencing Competitor Technologies

MLPA
Multiplex Ligation-dependent Probe Amplification (MLPA) is a competitor as it provides an alternative method for detecting copy number variations, which is one use case of Sanger sequencing.
next-generation sequencing
next-generation sequencing
Next-generation sequencing (NGS) is a high-throughput sequencing technology that can sequence millions of DNA fragments simultaneously, making it a direct competitor to Sanger sequencing for many applications. It is often faster and cheaper for large sequencing projects.
microarray
microarray
Microarrays can be used for detecting sequence variations, especially in targeted regions. Thus, it is a competitor for certain applications of Sanger Sequencing.
NGS
NGS
NGS (Next-Generation Sequencing) is a high-throughput sequencing technology that can sequence millions of DNA fragments simultaneously, making it a direct competitor to Sanger sequencing for many applications. It is often faster and cheaper for large sequencing projects.
Next Generation Sequencing (NGS)
Next Generation Sequencing (NGS)
NGS (Next-Generation Sequencing) is a high-throughput sequencing technology that can sequence millions of DNA fragments simultaneously, making it a direct competitor to Sanger sequencing for many applications. It is often faster and cheaper for large sequencing projects.
Number of organizations that mention technology
498
Sanger sequencing
126
MLPA
1,957
next-generation sequencing
259
microarray
2,504
NGS
679
Next Generation Sequencing (NGS)
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Sanger sequencing Complementary Technologies

fragment analysis
Fragment analysis, often performed via capillary electrophoresis, is used to analyze the results of Sanger sequencing. It helps determine the size and quantity of DNA fragments generated during the sequencing process.
PCR
PCR
PCR (Polymerase Chain Reaction) is a necessary upstream step for Sanger sequencing. It amplifies the DNA region of interest before sequencing.
capillary electrophoresis
capillary electrophoresis
Capillary electrophoresis is the standard method used to separate and detect the DNA fragments generated in Sanger sequencing.
Number of organizations that mention technology
498
Sanger sequencing
95
fragment analysis
5,114
PCR
576
capillary electrophoresis
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Which job functions commonly mention Sanger sequencing?

Geneticist
2.6% of all Geneticist jobs mention Sanger sequencing
View 16 jobs on Sumble
Research Assistant
0.1% of all Research Assistant jobs mention Sanger sequencing
View 204 jobs on Sumble
Scientist
0.1% of all Scientist jobs mention Sanger sequencing
View 363 jobs on Sumble
Laboratory Services
0.1% of all Laboratory Services jobs mention Sanger sequencing
View 290 jobs on Sumble
Researcher
0.1% of all Researcher jobs mention Sanger sequencing
View 335 jobs on Sumble
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Which organizations are mentioning Sanger sequencing?

Sanger sequencing
Synnovis
 
23 team
mention Sanger sequencing
↗
 5 people
use Sanger sequencing
↗
Sanger sequencing
Moderna
Manufacturing 
19 team
mention Sanger sequencing
↗
 6 people
use Sanger sequencing
↗
Sanger sequencing
Thermo Fisher Scientific
Scientific and Technical Services 
18 team
mention Sanger sequencing
↗
 29 people
use Sanger sequencing
↗
Sanger sequencing
Sanofi
Manufacturing 
18 team
mention Sanger sequencing
↗
 3 people
use Sanger sequencing
↗
Sanger sequencing
The University of Michigan
Educational Services 
16 team
mention Sanger sequencing
↗
 2 people
use Sanger sequencing
↗
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