Sanger sequencing Insights

What is Sanger sequencing?

Sanger sequencing, also known as chain-termination sequencing, is a method for determining the nucleotide sequence of DNA. It involves synthesizing a new DNA strand complementary to the template strand, but incorporating modified nucleotides (dideoxynucleotides) that terminate DNA synthesis. These terminated fragments are then separated by size, allowing the sequence to be read. It is commonly used for confirming DNA sequences, sequencing individual genes or small genomes, and for mutation detection.

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What other technologies are related to Sanger sequencing?

Sanger sequencing Competitor Technologies

MLPA

Multiplex Ligation-dependent Probe Amplification (MLPA) is a competitor as it provides an alternative method for detecting copy number variations, which is one use case of Sanger sequencing.

mentioned alongside Sanger sequencing in 31% (156) of relevant job posts

next-generation sequencing

Next-generation sequencing (NGS) is a high-throughput sequencing technology that can sequence millions of DNA fragments simultaneously, making it a direct competitor to Sanger sequencing for many applications. It is often faster and cheaper for large sequencing projects.

mentioned alongside Sanger sequencing in 5% (606) of relevant job posts

microarray

Microarrays can be used for detecting sequence variations, especially in targeted regions. Thus, it is a competitor for certain applications of Sanger Sequencing.

mentioned alongside Sanger sequencing in 13% (117) of relevant job posts

NGS

NGS (Next-Generation Sequencing) is a high-throughput sequencing technology that can sequence millions of DNA fragments simultaneously, making it a direct competitor to Sanger sequencing for many applications. It is often faster and cheaper for large sequencing projects.

mentioned alongside Sanger sequencing in 2% (291) of relevant job posts

Next Generation Sequencing (NGS)

NGS (Next-Generation Sequencing) is a high-throughput sequencing technology that can sequence millions of DNA fragments simultaneously, making it a direct competitor to Sanger sequencing for many applications. It is often faster and cheaper for large sequencing projects.

mentioned alongside Sanger sequencing in 4% (86) of relevant job posts

Sanger sequencing Complementary Technologies

fragment analysis

Fragment analysis, often performed via capillary electrophoresis, is used to analyze the results of Sanger sequencing. It helps determine the size and quantity of DNA fragments generated during the sequencing process.

mentioned alongside Sanger sequencing in 44% (129) of relevant job posts

PCR

PCR (Polymerase Chain Reaction) is a necessary upstream step for Sanger sequencing. It amplifies the DNA region of interest before sequencing.

mentioned alongside Sanger sequencing in 2% (817) of relevant job posts

capillary electrophoresis

Capillary electrophoresis is the standard method used to separate and detect the DNA fragments generated in Sanger sequencing.

mentioned alongside Sanger sequencing in 4% (118) of relevant job posts