Tech Insights

Next Generation Sequencing (NGS)

Last updated May 16, 2025, generated by Sumble

What is Next Generation Sequencing (NGS)?

Next-generation sequencing (NGS) technologies are a collection of high-throughput DNA sequencing technologies. These technologies allow for massively parallel sequencing of DNA or RNA molecules, enabling researchers to analyze large portions of the genome at once. NGS is commonly used in various applications, including genome sequencing, exome sequencing, transcriptome analysis (RNA-Seq), metagenomics, and targeted sequencing, allowing for the detection of genetic variations, gene expression patterns, and other genomic features.

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What other technologies are related to Next Generation Sequencing (NGS)?

Next Generation Sequencing (NGS) Competitor Technologies

Sanger sequencing

Sanger sequencing is an older sequencing technology that competes with NGS for certain applications, particularly for sequencing single genes or short stretches of DNA where high throughput is not required.

mentioned alongside Next Generation Sequencing (NGS) in 5% (86) of relevant job posts

Illumina

Illumina is a major provider of NGS platforms, and competes with other NGS technologies. In this case it might be considered a specific instance of NGS.

mentioned alongside Next Generation Sequencing (NGS) in 3% (79) of relevant job posts

Next Generation Sequencing (NGS) Complementary Technologies

PCR

PCR is often used to amplify DNA fragments before NGS library preparation, making it a complementary technology.

mentioned alongside Next Generation Sequencing (NGS) in 1% (305) of relevant job posts

qPCR

qPCR is used for quantifying DNA and RNA, and can be used to validate NGS results or prepare samples for NGS, thus it complements NGS.

mentioned alongside Next Generation Sequencing (NGS) in 1% (167) of relevant job posts

real-time PCR

Real-time PCR (qPCR) is used for quantifying DNA and RNA, and can be used to validate NGS results or prepare samples for NGS, thus it complements NGS.

mentioned alongside Next Generation Sequencing (NGS) in 2% (68) of relevant job posts