RNA isolation is a process used to purify RNA from a biological sample. This involves disrupting cells or tissues, removing proteins and DNA, and then selectively capturing and purifying the RNA. Common methods include liquid-liquid extraction (e.g., using TRIzol), column-based purification (e.g., using silica membranes), and magnetic bead-based separation. Isolated RNA is crucial for downstream applications such as RT-PCR, RNA sequencing, Northern blotting, and microarray analysis.
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