Tech Insights
RT-PCR

RT-PCR

Last updated , generated by Sumble
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What is RT-PCR?

RT-PCR, or Reverse Transcription Polymerase Chain Reaction, is a laboratory technique combining reverse transcription of RNA into DNA and amplification of specific DNA targets using PCR. It is widely used to quantify RNA levels, detect the presence of specific RNA viruses (like SARS-CoV-2), and study gene expression.

What other technologies are related to RT-PCR?

RT-PCR Competitor Technologies

PCR is a competitor to RT-PCR for DNA amplification, although RT-PCR specifically amplifies RNA.
mentioned alongside RT-PCR in 8% (3.4k) of relevant job posts
Northern blotting is a competitor for detecting RNA expression but is not amplification-based like RT-PCR.
mentioned alongside RT-PCR in 81% (299) of relevant job posts

RT-PCR Complementary Technologies

Western blotting can be used to confirm protein expression changes suggested by RT-PCR results.
mentioned alongside RT-PCR in 15% (1.6k) of relevant job posts
RNA is the starting material for RT-PCR and is thus complementary.
mentioned alongside RT-PCR in 18% (1.1k) of relevant job posts
Western blotting is complementary and can be used to confirm protein expression changes suggested by RT-PCR results.
mentioned alongside RT-PCR in 9% (1k) of relevant job posts

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