qRT-PCR Insights

What is qRT-PCR?

qRT-PCR, or quantitative reverse transcription PCR (also known as real-time RT-PCR), is a laboratory technique combining reverse transcription of RNA into DNA and amplification of specific DNA targets using polymerase chain reaction (PCR) to quantify the abundance of specific RNA molecules. It is widely used for gene expression analysis, viral load quantification, and other applications where quantifying RNA levels is important.

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What other technologies are related to qRT-PCR?

qRT-PCR Competitor Technologies

RNAseq

RNA sequencing (RNA-seq) is a high-throughput method for measuring the expression levels of all genes in a sample. It is a competitor to qRT-PCR which can only measure one or a few genes at a time.

mentioned alongside qRT-PCR in 4% (104) of relevant job posts

RNA-seq

RNA sequencing (RNA-seq) is a high-throughput method for measuring the expression levels of all genes in a sample. It is a competitor to qRT-PCR which can only measure one or a few genes at a time.

mentioned alongside qRT-PCR in 2% (120) of relevant job posts

RNA sequencing

RNA sequencing (RNA-seq) is a high-throughput method for measuring the expression levels of all genes in a sample. It is a competitor to qRT-PCR which can only measure one or a few genes at a time.

mentioned alongside qRT-PCR in 4% (70) of relevant job posts

qRT-PCR Complementary Technologies

Western blot

Western blot is used to detect protein expression, which can complement qRT-PCR data that quantifies mRNA levels. Used together, you can infer if differences in mRNA levels cause changes in protein levels.

mentioned alongside qRT-PCR in 5% (567) of relevant job posts

western blotting

Western blotting is used to detect protein expression, which can complement qRT-PCR data that quantifies mRNA levels. Used together, you can infer if differences in mRNA levels cause changes in protein levels.

mentioned alongside qRT-PCR in 5% (494) of relevant job posts

ELISA

ELISA is used to quantify protein expression levels, which can be compared to mRNA levels measured by qRT-PCR to assess translational efficiency.

mentioned alongside qRT-PCR in 2% (733) of relevant job posts