RNA-seq Insights

What is RNA-seq?

RNA-seq (RNA sequencing) is a sequencing technique which uses next-generation sequencing (NGS) to reveal the presence and quantity of RNA in a biological sample at a given moment in time. It is used to analyze the transcriptome, allowing researchers to study gene expression, discover new transcripts, and identify alternative splicing events. Common applications include comparing gene expression between different conditions, identifying biomarkers, and studying the effects of drug treatments.

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What other technologies are related to RNA-seq?

RNA-seq Complementary Technologies

ChIP-seq

Chromatin immunoprecipitation sequencing identifies DNA binding sites of proteins, informing on transcriptional regulation that influences RNA expression measured by RNA-seq.

mentioned alongside RNA-seq in 59% (1.4k) of relevant job posts

ATAC-seq

Assay for Transposase-Accessible Chromatin using sequencing maps regions of open chromatin, which influences gene transcription and thus RNA levels detected by RNA-seq.

mentioned alongside RNA-seq in 55% (1.2k) of relevant job posts

scRNA-seq

Single-cell RNA sequencing measures RNA expression in individual cells, providing higher resolution data compared to bulk RNA-seq, which averages expression across all cells.

mentioned alongside RNA-seq in 29% (363) of relevant job posts