scRNA-seq Insights

What is scRNA-seq?

Single-cell RNA sequencing (scRNA-seq) is a high-throughput method used to analyze the gene expression profiles of individual cells. It involves isolating single cells, lysing them to extract mRNA, converting the mRNA to cDNA, amplifying the cDNA, and then sequencing it using next-generation sequencing technologies. scRNA-seq is commonly used to study cellular heterogeneity, identify rare cell types, discover novel biomarkers, and understand developmental processes and disease mechanisms.

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What other technologies are related to scRNA-seq?

scRNA-seq Competitor Technologies

spatial transcriptomics

Spatial transcriptomics profiles gene expression while retaining spatial information, providing an alternative approach to understanding cellular heterogeneity in tissues.

mentioned alongside scRNA-seq in 8% (209) of relevant job posts

scRNA-seq Complementary Technologies

scATAC-seq

Single-cell ATAC-seq measures chromatin accessibility at the single-cell level, providing complementary information to scRNA-seq about gene regulation.

mentioned alongside scRNA-seq in 87% (279) of relevant job posts

CITE-seq

CITE-seq combines antibody-based protein detection with scRNA-seq, offering simultaneous measurement of RNA and protein expression at the single-cell level.

mentioned alongside scRNA-seq in 34% (143) of relevant job posts