Single-cell RNA sequencing (scRNA-seq) is a high-throughput method used to analyze the gene expression profiles of individual cells. It involves isolating single cells, lysing them to extract mRNA, converting the mRNA to cDNA, amplifying the cDNA, and then sequencing it using next-generation sequencing technologies. scRNA-seq is commonly used to study cellular heterogeneity, identify rare cell types, discover novel biomarkers, and understand developmental processes and disease mechanisms.
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