CITE-seq (Cellular Indexing of Transcriptomes and Epitopes by Sequencing) is a method that allows simultaneous measurement of gene expression and protein abundance in single cells. It combines traditional RNA sequencing with antibody-derived tags that are conjugated to oligonucleotides. These tags bind to specific cell surface proteins, and their abundance can be quantified along with mRNA levels using next-generation sequencing. CITE-seq is commonly used to gain a more comprehensive understanding of cellular identity and function, study immune cell populations, and investigate cellular heterogeneity.
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