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digital PCR

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**digital PCR**

What is digital PCR?

Digital PCR (dPCR) is a method for direct quantification of nucleic acids. Unlike traditional PCR, dPCR partitions the sample into many individual, separate reactions, where a single molecule is amplified. After PCR, the fraction of negative partitions is used to determine the absolute number of target molecules in the sample. It is commonly used for rare allele detection, copy number variation analysis, and precise quantification of nucleic acids.

Find 330 organizations using digital PCR on Sumble →

What other technologies are related to digital PCR?

digital PCR Competitor Technologies

multiplex PCR
Multiplex PCR amplifies multiple targets simultaneously, offering an alternative to dPCR for target quantification or detection. Digital PCR provides absolute quantification, while multiplex PCR offers higher throughput for relative quantification, making them competitors in some applications.
qPCR
qPCR is a real-time PCR method used for quantitative analysis of DNA or RNA. While dPCR offers absolute quantification, qPCR relies on relative quantification, making them competitors in applications requiring nucleic acid quantification.
NGS
NGS can be used for quantification and detection of DNA and RNA, providing an alternative to dPCR, particularly when analyzing many different sequences simultaneously. dPCR is useful for highly accurate quantification of specific targets, while NGS provides broader sequence information.
next-generation sequencing
Next-generation sequencing can be used for quantification and detection of DNA and RNA, providing an alternative to dPCR, particularly when analyzing many different sequences simultaneously. dPCR is useful for highly accurate quantification of specific targets, while NGS provides broader sequence information.
real-time PCR
Real-time PCR (qPCR) is used for quantitative analysis of DNA or RNA. While dPCR offers absolute quantification, qPCR relies on relative quantification, making them competitors in applications requiring nucleic acid quantification.
quantitative PCR
Quantitative PCR (qPCR) is used for quantitative analysis of DNA or RNA. While dPCR offers absolute quantification, qPCR relies on relative quantification, making them competitors in applications requiring nucleic acid quantification.
RT-qPCR
RT-qPCR is a real-time PCR method used for quantifying RNA. While dPCR offers absolute quantification, RT-qPCR relies on relative quantification, making them competitors in applications requiring RNA quantification.
Number of organizations that mention technology
330
digital PCR
77
multiplex PCR
3,157
qPCR
2,504
NGS
1,957
next-generation sequencing
978
real-time PCR
458
quantitative PCR
820
RT-qPCR
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digital PCR Complementary Technologies

DNA/RNA purification
DNA/RNA purification is a necessary step prior to digital PCR to ensure the quality and purity of the nucleic acid template. It improves the accuracy and reliability of dPCR results.
PCR
PCR is the fundamental process used in digital PCR to amplify DNA or RNA templates. dPCR relies on PCR to generate multiple copies of the target sequence within partitions.
RT-PCR
RT-PCR (Reverse Transcription PCR) is a type of PCR that is used for amplifying RNA, where the RNA is first converted to cDNA by reverse transcription. This cDNA can be used in Digital PCR to quantify the RNA's expression.
Number of organizations that mention technology
330
digital PCR
139
DNA/RNA purification
5,114
PCR
1,298
RT-PCR
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Which job functions commonly mention digital PCR?

Biologist
0.1% of all Biologist jobs mention digital PCR
View 58 jobs on Sumble
Scientist
0.1% of all Scientist jobs mention digital PCR
View 319 jobs on Sumble
Research Assistant
0.1% of all Research Assistant jobs mention digital PCR
View 114 jobs on Sumble
Researcher
0.1% of all Researcher jobs mention digital PCR
View 227 jobs on Sumble
Laboratory Services
87 Laboratory Services jobs mention digital PCR
View 87 jobs on Sumble
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Which organizations are mentioning digital PCR?

digital PCR
Merck
Health Care and Social Assistance 
21 team
mention digital PCR
↗
 2 people
use digital PCR
↗
digital PCR
Roche
Health Care and Social Assistance 
19 team
mention digital PCR
↗
 10 people
use digital PCR
↗
digital PCR
Bio-Rad Laboratories, Inc.
Manufacturing 
17 team
mention digital PCR
↗
 14 people
use digital PCR
↗
digital PCR
QIAGEN
Manufacturing 
15 team
mention digital PCR
↗
 10 people
use digital PCR
↗
digital PCR
Natera
Health Care and Social Assistance 
15 team
mention digital PCR
↗
 3 people
use digital PCR
↗
See more or filter by date, location, industry, etc →
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