multiplex PCR Insights

What is multiplex PCR?

Multiplex PCR is a variant of the polymerase chain reaction (PCR) that allows for simultaneous amplification of multiple target sequences in a single reaction. Instead of using one pair of primers to amplify a single region of DNA, multiplex PCR uses several pairs of primers to amplify different DNA sequences concurrently. This is commonly used in pathogen identification, forensic analysis, and high-throughput screening, allowing for faster and more efficient analysis compared to running multiple singleplex PCR reactions.

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What other technologies are related to multiplex PCR?

multiplex PCR Competitor Technologies

digital PCR

Digital PCR is a competitor because it offers an alternative method for nucleic acid quantification, providing absolute quantification without the need for standard curves, which can sometimes be an advantage over multiplex PCR.

mentioned alongside multiplex PCR in 5% (59) of relevant job posts

NGS

NGS is a competitor because it offers a broader, more comprehensive approach to analyzing genetic material, allowing for the simultaneous detection of many targets and novel variants, potentially replacing the need for targeted multiplex PCR assays in some applications.

mentioned alongside multiplex PCR in 0% (59) of relevant job posts

multiplex PCR Complementary Technologies

qPCR

qPCR is complementary as it can be used to validate and quantify the results obtained from multiplex PCR. It can also be used in conjunction with multiplex PCR for higher throughput analysis.

mentioned alongside multiplex PCR in 0% (103) of relevant job posts