What is immunoblot?

Immunoblotting, also known as Western blotting, is a widely used analytical technique in molecular biology and immunology. It is used to detect specific proteins in a sample of tissue homogenate or extract. The process involves separating proteins by gel electrophoresis, transferring them to a membrane (typically nitrocellulose or PVDF), and then probing the membrane with specific antibodies to visualize the protein of interest. It's commonly used for protein identification, quantification, and studying protein-protein interactions.