SDS-PAGE (Sodium Dodecyl-Sulfate Polyacrylamide Gel Electrophoresis) is a technique widely used in biochemistry, molecular biology, genetics, and proteomics to separate proteins according to their electrophoretic mobility (a function of length of polypeptide chain or molecular weight). The technique employs a discontinuous polyacrylamide gel as a support medium and sodium dodecyl sulfate (SDS) to denature the proteins and impart a negative charge proportional to their mass. It is commonly used to analyze protein samples, estimate protein molecular weights, assess protein purity, and monitor protein expression.
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