SDS-PAGE Insights

What is SDS-PAGE?

SDS-PAGE (Sodium Dodecyl-Sulfate Polyacrylamide Gel Electrophoresis) is a technique widely used in biochemistry, molecular biology, genetics, and proteomics to separate proteins according to their electrophoretic mobility (a function of length of polypeptide chain or molecular weight). The technique employs a discontinuous polyacrylamide gel as a support medium and sodium dodecyl sulfate (SDS) to denature the proteins and impart a negative charge proportional to their mass. It is commonly used to analyze protein samples, estimate protein molecular weights, assess protein purity, and monitor protein expression.

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What other technologies are related to SDS-PAGE?

SDS-PAGE Competitor Technologies

IEF

Isoelectric focusing (IEF) separates proteins based on their isoelectric point (pI), offering an alternative to size-based separation with SDS-PAGE.

mentioned alongside SDS-PAGE in 58% (350) of relevant job posts

SEC

Size-exclusion chromatography (SEC) separates proteins based on their size, similarly to SDS-PAGE, making it a direct competitor. SEC typically separates under native conditions while SDS-PAGE is denaturing.

mentioned alongside SDS-PAGE in 20% (584) of relevant job posts

CE-SDS

Capillary electrophoresis sodium dodecyl sulfate (CE-SDS) is a capillary electrophoresis technique used for size-based separation of proteins under denaturing conditions, similar to SDS-PAGE.

mentioned alongside SDS-PAGE in 26% (429) of relevant job posts

SEC-HPLC

Size-exclusion chromatography coupled with HPLC (SEC-HPLC) separates proteins based on their size. This is similar to SEC so, is a competitor.

mentioned alongside SDS-PAGE in 44% (120) of relevant job posts

native-PAGE

Native-PAGE separates proteins based on their size and charge under native (non-denaturing) conditions, similar to SDS-PAGE but without denaturation. Therefore, it's an alternative protein separation method.

mentioned alongside SDS-PAGE in 78% (63) of relevant job posts

size exclusion chromatography

See 'SEC'. (duplicate entry)

mentioned alongside SDS-PAGE in 28% (147) of relevant job posts

cIEF

Capillary isoelectric focusing (cIEF) is a form of IEF performed in capillaries, which separates proteins based on pI. Like IEF, it's a competitor.

mentioned alongside SDS-PAGE in 21% (195) of relevant job posts

icIEF

icIEF (imaging Capillary Isoelectric Focusing) is a capillary electrophoresis method that separates proteins based on isoelectric point (pI).

mentioned alongside SDS-PAGE in 19% (196) of relevant job posts

SDS-PAGE Complementary Technologies

Western blot

Western blot is commonly used after SDS-PAGE to confirm the identity of the protein separated by size.

mentioned alongside SDS-PAGE in 17% (1.9k) of relevant job posts

western blotting

See 'Western blot'. (duplicate entry)

mentioned alongside SDS-PAGE in 8% (856) of relevant job posts

SEC-MALS

Size-exclusion chromatography coupled with multi-angle light scattering (SEC-MALS) can be used to determine the absolute molecular weight of a protein, even those separated by SDS-PAGE.

mentioned alongside SDS-PAGE in 24% (175) of relevant job posts