cIEF Insights | Sumble

What is cIEF?

cIEF stands for capillary Isoelectric Focusing. It is a high-resolution analytical technique used to separate amphoteric molecules, primarily proteins, based on their isoelectric point (pI). The separation occurs within a capillary tube filled with ampholytes, which establish a pH gradient under an electric field. Proteins migrate until they reach the pH region corresponding to their pI, where they become neutral and focused into sharp bands. cIEF is commonly used for protein characterization, quality control, and biomarker discovery due to its ability to resolve closely related protein isoforms.

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What other technologies are related to cIEF?

cIEF Competitor Technologies

CE-SDS

CE-SDS is a capillary electrophoresis technique used for protein separation by size, similarly to cIEF which separates by pI.

mentioned alongside cIEF in 24% (397) of relevant job posts

CGE

CGE is a general term for capillary gel electrophoresis, a technique that, like cIEF, uses capillary electrophoresis to separate molecules, though by size.

mentioned alongside cIEF in 17% (74) of relevant job posts

CE

CE is the general platform that cIEF utilizes. Other modes of CE such as free solution electrophoresis may be alternatives to cIEF depending on the application.

mentioned alongside cIEF in 4% (218) of relevant job posts

SDS-PAGE

SDS-PAGE is a gel electrophoresis technique used for protein separation by size and can reveal information on protein purity and heterogeneity. It serves a similar purpose to cIEF for assessing protein identity and purity.

mentioned alongside cIEF in 3% (195) of relevant job posts

capillary electrophoresis

Capillary electrophoresis is the general platform that cIEF utilizes. Other modes of CE such as free solution electrophoresis may be alternatives to cIEF depending on the application.

mentioned alongside cIEF in 4% (116) of relevant job posts