Size-exclusion chromatography (SEC), also known as gel permeation chromatography (GPC) or gel filtration chromatography (GFC), is a chromatographic method in which molecules in solution are separated by their size, and in some cases molecular weight or hydrodynamic volume. It is commonly used to separate biomolecules such as proteins and polymers. SEC-HPLC uses HPLC (High-Performance Liquid Chromatography) instrumentation for higher resolution and faster analysis times.
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