SEC-HPLC Insights

What is SEC-HPLC?

Size-exclusion chromatography (SEC), also known as gel permeation chromatography (GPC) or gel filtration chromatography (GFC), is a chromatographic method in which molecules in solution are separated by their size, and in some cases molecular weight or hydrodynamic volume. It is commonly used to separate biomolecules such as proteins and polymers. SEC-HPLC uses HPLC (High-Performance Liquid Chromatography) instrumentation for higher resolution and faster analysis times.

Find organizations using SEC-HPLC on Sumble →

What other technologies are related to SEC-HPLC?

SEC-HPLC Competitor Technologies

RP-HPLC

RP-HPLC separates molecules based on hydrophobicity, while SEC-HPLC separates based on size. They are both HPLC techniques used for separation and purification of molecules, making them alternatives in some applications.

mentioned alongside SEC-HPLC in 14% (85) of relevant job posts

SEC-HPLC Complementary Technologies

SDS-PAGE

SDS-PAGE separates proteins based on size and charge, providing information on protein purity and molecular weight. It is often used in conjunction with SEC-HPLC to analyze the fractions obtained, assessing the homogeneity and size distribution of the separated components.

mentioned alongside SEC-HPLC in 2% (121) of relevant job posts

CE-SDS

CE-SDS is a capillary electrophoresis technique that separates proteins based on size and charge, similar to SDS-PAGE but often with higher resolution and automation. It can be used to analyze SEC-HPLC fractions to determine their purity and size distribution, or to characterize the sample before SEC-HPLC.

mentioned alongside SEC-HPLC in 4% (62) of relevant job posts