RP-HPLC Insights

What is RP-HPLC?

Reversed-phase high-performance liquid chromatography (RP-HPLC) is a chromatographic technique used to separate non-polar molecules. It uses a non-polar stationary phase and a polar mobile phase. It's widely used in pharmaceutical analysis, environmental monitoring, food science, and biochemistry to identify, quantify, and purify compounds.

Find organizations using RP-HPLC on Sumble →

What other technologies are related to RP-HPLC?

RP-HPLC Competitor Technologies

SEC-HPLC

SEC-HPLC separates molecules based on size, like RP-HPLC separates based on hydrophobicity, making them alternative separation techniques.

mentioned alongside RP-HPLC in 31% (84) of relevant job posts

SEC

SEC separates molecules based on size, like RP-HPLC separates based on hydrophobicity, making them alternative separation techniques.

mentioned alongside RP-HPLC in 9% (262) of relevant job posts

HIC

HIC separates proteins based on hydrophobicity, similar to RP-HPLC, making them alternative separation techniques.

mentioned alongside RP-HPLC in 14% (133) of relevant job posts

IEX

IEX separates molecules based on charge, offering an alternative separation mechanism to RP-HPLC's hydrophobicity-based separation.

mentioned alongside RP-HPLC in 3% (144) of relevant job posts

UPLC

UPLC is a more efficient form of HPLC. It performs the same separation mechanism as RP-HPLC, but can run at higher pressures, thus it is a competitor.

mentioned alongside RP-HPLC in 1% (102) of relevant job posts

capillary electrophoresis

Capillary electrophoresis separates molecules based on their charge and size, offering an alternative separation mechanism to RP-HPLC's hydrophobicity-based separation.

mentioned alongside RP-HPLC in 2% (54) of relevant job posts

HPLC

HPLC is a general term for high-performance liquid chromatography. RP-HPLC is a specific type of HPLC, and other types of HPLC are competitors for certain applications.

mentioned alongside RP-HPLC in 0% (184) of relevant job posts

CE

CE separates molecules based on charge and size, offering an alternative separation mechanism to RP-HPLC's hydrophobicity-based separation.

mentioned alongside RP-HPLC in 1% (54) of relevant job posts

RP-HPLC Complementary Technologies

CE-SDS

CE-SDS is used to determine protein purity and molecular weight, providing information that complements RP-HPLC analysis.

mentioned alongside RP-HPLC in 7% (121) of relevant job posts

AUC

AUC is an orthogonal method that measures the size and shape of macromolecules in solution, which can be used to validate or complement RP-HPLC results.

mentioned alongside RP-HPLC in 12% (55) of relevant job posts

cIEF

cIEF separates proteins based on their isoelectric point, a different property than hydrophobicity, offering complementary information to RP-HPLC.

mentioned alongside RP-HPLC in 8% (75) of relevant job posts