HPLC Insights | Sumble

What is HPLC?

High-Performance Liquid Chromatography (HPLC) is an analytical technique used to separate, identify, and quantify each component in a mixture. It works by passing a liquid mobile phase containing the sample through a column packed with a solid stationary phase. Different components of the sample interact differently with the stationary phase, causing them to elute at different times, allowing for their separation and quantification.

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What other technologies are related to HPLC?

HPLC Competitor Technologies

GC

Gas Chromatography is a separation technique used for volatile compounds, while HPLC is for non-volatile or thermally labile compounds. Thus, GC can be seen as a competitor to HPLC in some applications.

mentioned alongside HPLC in 74% (23.7k) of relevant job posts

GC-MS

Gas Chromatography-Mass Spectrometry combines the separation of GC with MS, and is used for volatile compounds, while HPLC is for non-volatile or thermally labile compounds.

mentioned alongside HPLC in 37% (4.5k) of relevant job posts

TLC

Thin Layer Chromatography is a simpler, less expensive separation technique that can be used for similar purposes to HPLC.

mentioned alongside HPLC in 79% (2k) of relevant job posts

IC

Ion Chromatography is a separation technique focused on ions and polar molecules, and can be used as an alternative to HPLC for some applications.

mentioned alongside HPLC in 50% (2.7k) of relevant job posts

SDS-PAGE

SDS-PAGE (Sodium Dodecyl-Sulfate Polyacrylamide Gel Electrophoresis) is a technique used to separate proteins based on their size, and can be used as an alternative separation technique to HPLC.

mentioned alongside HPLC in 37% (2.3k) of relevant job posts

GCMS

Gas Chromatography-Mass Spectrometry combines the separation of GC with MS, and is used for volatile compounds, while HPLC is for non-volatile or thermally labile compounds.

mentioned alongside HPLC in 41% (1.4k) of relevant job posts

CE

Capillary Electrophoresis is a separation technique based on the movement of ions in an electric field, and can be a competitor to HPLC.

mentioned alongside HPLC in 31% (1.7k) of relevant job posts