SEC-MALS, or Size Exclusion Chromatography with Multi-Angle Light Scattering, is a powerful analytical technique used to determine the absolute molecular weight and size of macromolecules in solution. It combines the separation capabilities of SEC with the direct measurement of light scattering. This allows for the determination of molecular weight, size (radius of gyration), and aggregation state of proteins, polymers, and nanoparticles without relying on column calibration or reference standards. It's commonly used for characterizing proteins, polymers, and other biomolecules in research and development, as well as quality control.
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