SEC-MALS Insights

What is SEC-MALS?

SEC-MALS, or Size Exclusion Chromatography with Multi-Angle Light Scattering, is a powerful analytical technique used to determine the absolute molecular weight and size of macromolecules in solution. It combines the separation capabilities of SEC with the direct measurement of light scattering. This allows for the determination of molecular weight, size (radius of gyration), and aggregation state of proteins, polymers, and nanoparticles without relying on column calibration or reference standards. It's commonly used for characterizing proteins, polymers, and other biomolecules in research and development, as well as quality control.

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What other technologies are related to SEC-MALS?

SEC-MALS Competitor Technologies

DLS

Dynamic Light Scattering (DLS) is used to measure the hydrodynamic size of particles in solution, similar to SEC-MALS, but often with less accuracy and resolution for complex mixtures.

mentioned alongside SEC-MALS in 12% (299) of relevant job posts

AUC

Analytical Ultracentrifugation (AUC) is another method for determining the size and shape of macromolecules in solution, offering similar information as SEC-MALS but using a different physical principle.

mentioned alongside SEC-MALS in 19% (93) of relevant job posts

SEC-MALS Complementary Technologies

HIC

Hydrophobic Interaction Chromatography (HIC) separates molecules based on hydrophobicity. It can be used upstream of SEC-MALS to purify the sample.

mentioned alongside SEC-MALS in 11% (107) of relevant job posts

SEC

Size Exclusion Chromatography (SEC) is the chromatographic separation technique used in SEC-MALS to separate molecules by size. SEC alone provides an estimate of molecular weight, while SEC-MALS provides absolute molecular weight measurements.

mentioned alongside SEC-MALS in 5% (153) of relevant job posts

RP-HPLC

Reverse Phase High-Performance Liquid Chromatography (RP-HPLC) separates molecules based on hydrophobicity and can be used for sample preparation prior to SEC-MALS analysis.

mentioned alongside SEC-MALS in 10% (60) of relevant job posts