X-ray crystallography Insights

What is X-ray crystallography?

X-ray crystallography is a technique used to determine the atomic and molecular structure of a crystal. In this technique, a beam of X-rays strikes a crystal and diffracts into many specific directions. By measuring the angles and intensities of these diffracted beams, a three-dimensional picture of the density of electrons within the crystal can be produced. From this electron density, the positions of the atoms in the crystal can be determined, as well as their chemical bonds, disorders, and other attributes. It is widely used in chemistry, materials science, and biology to identify the structure of a wide range of materials, including proteins, DNA, and pharmaceuticals.

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What other technologies are related to X-ray crystallography?

X-ray crystallography Competitor Technologies

cryo-EM

Cryo-EM is an alternative structural biology technique that can often solve structures where X-ray crystallography struggles, especially for large or flexible molecules.

mentioned alongside X-ray crystallography in 35% (435) of relevant job posts

cryo-electron microscopy

Cryo-electron microscopy (cryo-EM) is an alternative structural biology technique that can often solve structures where X-ray crystallography struggles, especially for large or flexible molecules.

mentioned alongside X-ray crystallography in 35% (150) of relevant job posts

cryoEM

CryoEM is an alternative structural biology technique that can often solve structures where X-ray crystallography struggles, especially for large or flexible molecules.

mentioned alongside X-ray crystallography in 26% (118) of relevant job posts

NMR spectroscopy

NMR spectroscopy is another structural biology technique that provides information about the structure and dynamics of molecules in solution, often used when X-ray crystallography is not feasible.

mentioned alongside X-ray crystallography in 14% (97) of relevant job posts

NMR

NMR is another structural biology technique that provides information about the structure and dynamics of molecules in solution, often used when X-ray crystallography is not feasible.

mentioned alongside X-ray crystallography in 2% (225) of relevant job posts

X-ray crystallography Complementary Technologies

ITC

Isothermal titration calorimetry (ITC) measures the heat released or absorbed during a binding event, providing thermodynamic parameters that complement structural information from X-ray crystallography.

mentioned alongside X-ray crystallography in 13% (112) of relevant job posts

SPR

Surface plasmon resonance (SPR) is used to study biomolecular interactions in real-time. It provides binding affinity and kinetic data which is complementary to the structural information obtained from X-ray crystallography.

mentioned alongside X-ray crystallography in 5% (153) of relevant job posts

DSF

Differential scanning fluorimetry (DSF) assesses protein stability, which is valuable information to know when optimizing crystallization conditions for X-ray crystallography.

mentioned alongside X-ray crystallography in 7% (61) of relevant job posts