What is cryoEM?

Cryo-electron microscopy (cryo-EM) is a form of transmission electron microscopy (TEM) where samples are studied at cryogenic temperatures (typically liquid nitrogen temperatures). Cryo-EM is commonly used to determine the structures of large biomolecules and macromolecular assemblies, such as proteins and viruses, in their native hydrated state. This technique avoids the need for crystallization, which can be challenging or impossible for some biological samples, and allows for the study of structures closer to their natural conformation.