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cryo-electron microscopy

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**cryo-electron microscopy**

What is cryo-electron microscopy?

Cryo-electron microscopy (cryo-EM) is a form of transmission electron microscopy (TEM) where samples are studied at cryogenic temperatures (typically liquid nitrogen temperatures). Cryo-EM is used to determine the 3D structures of biological macromolecules, such as proteins and nucleic acids, at near-atomic resolution. Samples are rapidly cooled (vitrified) to preserve their native structure within a thin film of amorphous ice, avoiding the formation of crystalline ice that can damage or distort the sample.

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