What is cryo-EM?

Cryo-electron microscopy (cryo-EM) is a form of transmission electron microscopy (TEM) where samples are studied at cryogenic temperatures (typically liquid nitrogen temperatures). Cryo-EM is often used to determine the three-dimensional structures of biological molecules, such as proteins and nucleic acids. Samples are rapidly cooled to vitrify them, preserving their native state and preventing the formation of ice crystals that can damage the structure.