western blots Insights

What is western blots?

Western blotting (also called immunoblotting) is a widely used analytical technique in molecular biology and immunology to detect specific proteins in a sample of tissue homogenate or extract. It involves separating proteins by gel electrophoresis, transferring them to a membrane, and then probing the membrane with labeled antibodies specific to the target protein. The technique allows researchers to identify and quantify the presence of a protein of interest, assess protein size, and examine protein expression levels.

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What other technologies are related to western blots?

western blots Competitor Technologies

ELISAs

ELISAs can provide similar protein quantification data as Western blots, but with higher throughput and different advantages/disadvantages.

mentioned alongside western blots in 15% (379) of relevant job posts

immunohistochemistry

Immunohistochemistry detects proteins in tissue sections, offering spatial information that Western blots lack, and can sometimes be used as an alternative method of protein detection.

mentioned alongside western blots in 5% (438) of relevant job posts

immunofluorescence

Immunofluorescence is a technique for visualizing proteins in cells, offering spatial information that Western blots lack, and can sometimes be used as an alternative method of protein detection.

mentioned alongside western blots in 5% (169) of relevant job posts

immunocytochemistry

Immunocytochemistry detects proteins in cells, offering spatial information that Western blots lack, and can sometimes be used as an alternative method of protein detection.

mentioned alongside western blots in 8% (87) of relevant job posts

immunostaining

Immunostaining is a technique for visualizing proteins in cells or tissues, offering spatial information that Western blots lack, and can sometimes be used as an alternative method of protein detection.

mentioned alongside western blots in 5% (75) of relevant job posts

western blots Complementary Technologies

immunoprecipitations

Immunoprecipitation is often used as a sample preparation step before Western blotting to enrich for the protein of interest.

mentioned alongside western blots in 39% (61) of relevant job posts

gel electrophoresis

Gel electrophoresis, specifically SDS-PAGE, is a crucial step in Western blotting for separating proteins by size.

mentioned alongside western blots in 4% (214) of relevant job posts

SDS-PAGE

SDS-PAGE (sodium dodecyl-sulfate polyacrylamide gel electrophoresis) is the gel electrophoresis technique used to separate proteins by size in Western blotting.

mentioned alongside western blots in 3% (183) of relevant job posts