immunohistochemistry Insights

What is immunohistochemistry?

Immunohistochemistry (IHC) is a technique used to visualize specific proteins or antigens in tissue sections by use of labeled antibodies that bind specifically to these antigens. IHC is widely used in diagnostic pathology to identify specific cell types, determine the distribution of proteins within a tissue, and detect abnormal cells, such as cancer cells.

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What other technologies are related to immunohistochemistry?

immunohistochemistry Competitor Technologies

in situ hybridization

In situ hybridization, like immunohistochemistry, is used to visualize the location of specific nucleic acid sequences within cells or tissues. While IHC detects proteins, ISH detects DNA or RNA. Therefore, they are competitors, but provide different information.

mentioned alongside immunohistochemistry in 66% (803) of relevant job posts

immunofluorescence

Immunofluorescence is very similar to immunohistochemistry, both use antibodies to detect specific antigens. The main difference is the method of detection (fluorescent dyes vs enzyme/substrate). Therefore, they are competitors.

mentioned alongside immunohistochemistry in 32% (1.1k) of relevant job posts

immunohistochemistry Complementary Technologies

western blotting

Western blotting is used to detect and quantify protein expression levels in a sample. It is complementary to immunohistochemistry, which provides information about the spatial distribution of proteins within tissues.

mentioned alongside immunohistochemistry in 16% (1.7k) of relevant job posts

Antibody production and purification

Antibodies are essential reagents for immunohistochemistry. Antibody production and purification are crucial for generating high-quality antibodies needed for IHC.

mentioned alongside immunohistochemistry in 89% (252) of relevant job posts

flow cytometry

Flow cytometry is a technique for analyzing cells based on their physical and chemical characteristics. It can be used to quantify protein expression, similar to IHC, but doesn't provide spatial information.

mentioned alongside immunohistochemistry in 8% (2.5k) of relevant job posts