in situ hybridization Insights

What is in situ hybridization?

In situ hybridization (ISH) is a type of molecular biology technique used to detect the presence or absence of specific DNA or RNA sequences in cells or tissues. It involves using a labeled complementary DNA or RNA strand (a probe) to hybridize with the sequence of interest within the cell or tissue. The probe is labeled with a fluorescent dye or enzyme, allowing visualization of the location of the target sequence under a microscope. It is commonly used in research and diagnostics to study gene expression, identify infectious agents, and detect chromosomal abnormalities.

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What other technologies are related to in situ hybridization?

in situ hybridization Competitor Technologies

immunohistochemistry

Immunohistochemistry uses antibodies to detect proteins in tissue sections, providing similar spatial information to in situ hybridization but targeting proteins instead of nucleic acids.

mentioned alongside in situ hybridization in 9% (803) of relevant job posts

northern blotting

Northern blotting detects specific RNA sequences, similar to in situ hybridization, but it does not provide spatial information as it is performed on RNA extracted from the tissue.

mentioned alongside in situ hybridization in 57% (113) of relevant job posts

Enzyme Histochemistry

Similar to immunohistochemistry and in situ hybridization, it uses enzymes to detect specific targets in tissue sections, providing spatial information.

mentioned alongside in situ hybridization in 36% (63) of relevant job posts

immunofluorescence

Immunofluorescence, like immunohistochemistry, uses fluorescently labeled antibodies to detect proteins in tissue sections, providing spatial information comparable to in situ hybridization but targeting proteins.

mentioned alongside in situ hybridization in 6% (226) of relevant job posts

immunocytochemistry

Immunocytochemistry uses antibodies to detect proteins in cells, providing similar information to in situ hybridization but targeting proteins instead of nucleic acids, with spatial resolution at the cellular level.

mentioned alongside in situ hybridization in 6% (67) of relevant job posts

spatial transcriptomics

Spatial transcriptomics offers an alternative approach to mapping gene expression within a tissue, often on a genome-wide scale, thereby providing spatial resolution of gene expression patterns.

mentioned alongside in situ hybridization in 2% (62) of relevant job posts

in situ hybridization Complementary Technologies

confocal microscopy

Confocal microscopy is often used to visualize and enhance the signal from in situ hybridization experiments, providing improved resolution and optical sectioning.

mentioned alongside in situ hybridization in 3% (115) of relevant job posts

microscopy

Microscopy is essential for visualizing the results of in situ hybridization, allowing for the detection and localization of the labeled nucleic acid probes within cells or tissues.

mentioned alongside in situ hybridization in 2% (142) of relevant job posts

histology

Histology provides the structural context for in situ hybridization, allowing for the localization of gene expression within specific tissue types and cell populations.

mentioned alongside in situ hybridization in 3% (54) of relevant job posts