TR-FRET Insights

What is TR-FRET?

Time-Resolved Förster Resonance Energy Transfer (TR-FRET) is a homogeneous assay technology commonly used in drug discovery and other biological research. It combines FRET technology with time-resolved fluorescence detection. A donor molecule with a long-lived excited state transfers energy to an acceptor molecule if they are in close proximity. The signal is measured after a delay, reducing interference from short-lived background fluorescence.

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What other technologies are related to TR-FRET?

TR-FRET Competitor Technologies

FP

Fluorescence Polarization (FP) is another fluorescence-based assay used for similar applications like binding assays, making it a direct competitor.

mentioned alongside TR-FRET in 18% (103) of relevant job posts

luminescence

Luminescence-based assays are used to measure molecular interactions and reporter gene expression and, as such, are a direct competitor for TR-FRET.

mentioned alongside TR-FRET in 14% (64) of relevant job posts

AlphaLISA

AlphaLISA is a bead-based proximity assay that, similar to TR-FRET, detects molecular interactions, making it a direct competitor.

mentioned alongside TR-FRET in 12% (69) of relevant job posts

ELISA

ELISA (Enzyme-Linked Immunosorbent Assay) is a plate-based assay used for detecting and quantifying substances like antibodies and proteins. While ELISA is typically used for quantification of proteins, it can be a competitor in certain applications where TR-FRET could be used similarly.

mentioned alongside TR-FRET in 0% (153) of relevant job posts

TR-FRET Complementary Technologies

ITC

Isothermal Titration Calorimetry (ITC) measures the heat released or absorbed during a binding event, providing thermodynamic information that complements the binding affinity data obtained from TR-FRET.

mentioned alongside TR-FRET in 7% (62) of relevant job posts

MST

Microscale Thermophoresis (MST) measures changes in fluorescence upon temperature gradients to quantify binding affinities. It provides orthogonal validation of TR-FRET results.

mentioned alongside TR-FRET in 5% (64) of relevant job posts

SPR

Surface Plasmon Resonance (SPR) is a label-free technology that measures binding kinetics and affinity. It offers complementary information to TR-FRET, which is an endpoint assay.

mentioned alongside TR-FRET in 3% (94) of relevant job posts