AlphaLISA Insights

What is AlphaLISA?

AlphaLISA (Amplified Luminescent Proximity Homogeneous Assay) is a bead-based, no-wash assay technology used for detecting and quantifying biomolecules in solution. It relies on the proximity of donor and acceptor beads, where excitation of the donor bead triggers a cascade of energy transfer leading to light emission from the acceptor bead. It is commonly used in drug discovery and life science research for applications like protein-protein interaction studies, enzyme activity assays, and biomarker detection.

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What other technologies are related to AlphaLISA?

AlphaLISA Competitor Technologies

HTRF

Homogeneous Time-Resolved Fluorescence (HTRF) is a competing homogeneous assay technology for biomolecular interaction studies.

mentioned alongside AlphaLISA in 22% (136) of relevant job posts

TR-FRET

Time-Resolved Fluorescence Resonance Energy Transfer (TR-FRET) is a competing homogeneous assay technology for biomolecular interaction studies, similar to AlphaLISA.

mentioned alongside AlphaLISA in 19% (69) of relevant job posts

MSD

Meso Scale Discovery (MSD) offers electrochemiluminescence-based assays, which compete in the multiplexed immunoassay space.

mentioned alongside AlphaLISA in 4% (162) of relevant job posts

Luminex

Luminex technology is a bead-based multiplexed assay platform that competes with AlphaLISA in high-throughput screening and immunoassay applications.

mentioned alongside AlphaLISA in 4% (115) of relevant job posts

ELISA

ELISA (Enzyme-Linked Immunosorbent Assay) is a widely used immunoassay that competes with AlphaLISA, though typically less amenable to high-throughput screening.

mentioned alongside AlphaLISA in 1% (386) of relevant job posts

SPR

Surface Plasmon Resonance (SPR) is a label-free technology used to measure biomolecular interactions. Although mechanistically different, it competes with AlphaLISA in some applications.

mentioned alongside AlphaLISA in 3% (89) of relevant job posts

AlphaLISA Complementary Technologies

flow cytometry

Flow cytometry is used to analyze cells, and can be used in combination with AlphaLISA to understand secreted factors.

mentioned alongside AlphaLISA in 0% (149) of relevant job posts

qPCR

qPCR (quantitative PCR) measures gene expression, while AlphaLISA measures protein levels. These can be used in combination to study biological pathways.

mentioned alongside AlphaLISA in 0% (98) of relevant job posts

Western blot

Western blot is a technique used to detect specific proteins in a sample. It provides orthogonal validation to AlphaLISA results.

mentioned alongside AlphaLISA in 1% (68) of relevant job posts