AlphaLISA (Amplified Luminescent Proximity Homogeneous Assay) is a bead-based, no-wash assay technology used for detecting and quantifying biomolecules in solution. It relies on the proximity of donor and acceptor beads, where excitation of the donor bead triggers a cascade of energy transfer leading to light emission from the acceptor bead. It is commonly used in drug discovery and life science research for applications like protein-protein interaction studies, enzyme activity assays, and biomarker detection.
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