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immunoblotting

immunoblotting

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What is immunoblotting?

Immunoblotting, also known as Western blotting, is a widely used analytical technique in molecular biology and immunology to detect specific proteins in a sample of tissue homogenate or extract. It involves separating proteins by gel electrophoresis, transferring them to a membrane (typically nitrocellulose or PVDF), and then probing the membrane with specific antibodies that bind to the target protein. The bound antibodies are then detected, often using enzyme-linked secondary antibodies or fluorescently labeled antibodies, allowing for the identification and quantification of the protein of interest. Immunoblotting is commonly used to confirm protein expression, determine protein size, and assess post-translational modifications.

What other technologies are related to immunoblotting?

immunoblotting Competitor Technologies

Immunohistochemistry detects proteins in tissue sections, offering an alternative approach to immunoblotting for protein detection and localization.
mentioned alongside immunoblotting in 3% (262) of relevant job posts
Flow cytometry detects proteins on or within single cells, providing an alternative method of protein detection.
mentioned alongside immunoblotting in 1% (445) of relevant job posts
Immunofluorescence detects proteins in cells, offering an alternative approach to immunoblotting for protein detection and localization.
mentioned alongside immunoblotting in 4% (126) of relevant job posts
ELISA is an alternative method for protein quantification.
mentioned alongside immunoblotting in 1% (369) of relevant job posts
Immunocytochemistry detects proteins in cells, offering an alternative approach to immunoblotting for protein detection and localization.
mentioned alongside immunoblotting in 5% (53) of relevant job posts
Confocal microscopy can be used to detect proteins in cells and tissues with high resolution, serving as an alternative to immunoblotting for protein detection and localization.
mentioned alongside immunoblotting in 2% (78) of relevant job posts
ELISAs are an alternative method for protein quantification.
mentioned alongside immunoblotting in 2% (57) of relevant job posts
FACS (Fluorescence-activated cell sorting) uses flow cytometry to sort cells based on protein expression, thus can serve as an alternative to immunoblotting.
mentioned alongside immunoblotting in 1% (56) of relevant job posts

immunoblotting Complementary Technologies

Immunoprecipitation is often used to enrich the protein of interest before immunoblotting.
mentioned alongside immunoblotting in 11% (167) of relevant job posts
SDS-PAGE is used to separate proteins by size prior to immunoblotting.
mentioned alongside immunoblotting in 2% (114) of relevant job posts
Gel electrophoresis is used to separate proteins prior to immunoblotting.
mentioned alongside immunoblotting in 2% (96) of relevant job posts

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