CUT&RUN Insights

What is CUT&RUN?

CUT&RUN (Cleavage Under Targets & Release Using Nuclease) is an epigenetic profiling strategy used to map DNA-protein interactions genome-wide. It uses antibody-targeted controlled cleavage by micrococcal nuclease (MNase) to selectively digest and release specific DNA fragments for sequencing. Compared to ChIP-seq, CUT&RUN offers higher resolution, lower background, and requires fewer cells.

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What other technologies are related to CUT&RUN?

CUT&RUN Competitor Technologies

CUT&Tag

CUT&Tag is a competitor to CUT&RUN as it is an alternative chromatin profiling method using antibody-guided enzymatic cleavage.

mentioned alongside CUT&RUN in 33% (90) of relevant job posts

ChIP-seq

ChIP-seq is a competitor to CUT&RUN as it is another method for mapping protein-DNA interactions, although CUT&RUN offers some advantages in terms of resolution and efficiency.

mentioned alongside CUT&RUN in 10% (242) of relevant job posts

CUT&RUN Complementary Technologies

ATAC-seq

ATAC-seq provides information about chromatin accessibility, which can complement CUT&RUN data that focuses on specific protein-DNA interactions. Combining ATAC-seq with CUT&RUN can provide a more comprehensive view of chromatin structure and regulation.

mentioned alongside CUT&RUN in 9% (205) of relevant job posts

next-generation sequencing

Next-generation sequencing (NGS) is the underlying technology used to read the DNA fragments generated in a CUT&RUN experiment. NGS is essential for analyzing the results of CUT&RUN.

mentioned alongside CUT&RUN in 1% (60) of relevant job posts

PCR

PCR may be used in the library preparation steps of CUT&RUN to amplify the DNA fragments prior to sequencing.

mentioned alongside CUT&RUN in 0% (59) of relevant job posts