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affinity chromatography

affinity chromatography

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What is affinity chromatography?

Affinity chromatography is a separation technique based on specific binding interactions between an immobilized ligand and a target molecule. The target molecule is selectively adsorbed to the ligand, while other molecules are washed away. The bound target is then eluted by disrupting the binding interaction, often by changing buffer conditions, thereby isolating the target molecule.

What other technologies are related to affinity chromatography?

affinity chromatography Competitor Technologies

Size exclusion chromatography separates molecules based on size, similar to affinity chromatography which separates based on specific binding affinity, offering an alternative separation method.
mentioned alongside affinity chromatography in 19% (98) of relevant job posts
Ion exchange chromatography separates molecules based on charge, an alternative to affinity chromatography's separation by binding affinity.
mentioned alongside affinity chromatography in 23% (62) of relevant job posts
SEC (Size Exclusion Chromatography) separates molecules based on size, similar to affinity chromatography which separates based on specific binding affinity, offering an alternative separation method.
mentioned alongside affinity chromatography in 3% (81) of relevant job posts
IEX (Ion Exchange Chromatography) separates molecules based on charge, an alternative to affinity chromatography's separation by binding affinity.
mentioned alongside affinity chromatography in 2% (70) of relevant job posts

affinity chromatography Complementary Technologies

SDS-PAGE is used to analyze the purity and molecular weight of proteins after affinity chromatography purification.
mentioned alongside affinity chromatography in 2% (139) of relevant job posts
ELISA can be used to confirm the presence and quantity of a target molecule purified by affinity chromatography.
mentioned alongside affinity chromatography in 0% (108) of relevant job posts
HPLC can be used to further purify and analyze the fractions obtained from affinity chromatography. It is also a common platform for affinity chromatography.
mentioned alongside affinity chromatography in 0% (110) of relevant job posts

Which job functions mention affinity chromatography?

Job function
Jobs mentioning affinity chromatography
Orgs mentioning affinity chromatography

Which organizations are mentioning affinity chromatography?

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