ion exchange chromatography Insights

What is ion exchange chromatography?

Ion exchange chromatography (IEC) is a separation technique that separates molecules based on their net surface charge. It is commonly used in water treatment, food and beverage analysis, pharmaceutical production, and biochemistry to purify and analyze ions and charged molecules like proteins and amino acids. The stationary phase carries an electrical charge, and molecules with an opposite charge are attracted to it. Molecules are then eluted by changing the ionic strength or pH of the mobile phase.

Find organizations using ion exchange chromatography on Sumble →

What other technologies are related to ion exchange chromatography?

ion exchange chromatography Competitor Technologies

size exclusion chromatography

Size exclusion chromatography separates molecules based on size, similar to how ion exchange separates based on charge. They both achieve separation of molecules, making them competitors.

mentioned alongside ion exchange chromatography in 18% (96) of relevant job posts

affinity chromatography

Affinity chromatography separates molecules based on highly specific binding interactions. While the interaction is different from charge (ion exchange), it achieves the same goal of separating and purifying specific target molecules from a mixture, which positions it as a competitor to ion exchange chromatography.

mentioned alongside ion exchange chromatography in 15% (62) of relevant job posts

ion exchange chromatography Complementary Technologies

SDS-PAGE

SDS-PAGE is used to separate proteins based on size under denaturing conditions. It is often used after ion exchange chromatography to assess the purity and molecular weight of the fractions obtained. Thus, it is a complementary technique.

mentioned alongside ion exchange chromatography in 1% (60) of relevant job posts