What is Sanger?

Sanger sequencing, also known as chain termination sequencing, is a method for determining the nucleotide sequence of DNA. It is widely used for sequencing relatively short DNA fragments, typically up to 900 base pairs, and is often employed for validating results from high-throughput sequencing methods. It involves synthesizing a new DNA strand complementary to the template strand using modified nucleotides (dideoxynucleotides) that terminate DNA elongation, resulting in fragments of varying lengths. These fragments are then separated by size using electrophoresis, allowing the sequence to be read.