HPLC-UV Insights

What is HPLC-UV?

HPLC-UV stands for High-Performance Liquid Chromatography with Ultraviolet-Visible detection. It is an analytical technique used to separate, identify, and quantify components in a liquid mixture. The HPLC system separates compounds based on their physical and chemical properties by passing them through a chromatography column. A UV-Vis detector measures the absorbance of the separated compounds as they elute from the column, providing quantitative data based on the Beer-Lambert Law.

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What other technologies are related to HPLC-UV?

HPLC-UV Competitor Technologies

ELISA

ELISA is a common analytical biochemistry assay to detect the presence of a substance, usually an antigen, in a biological sample. It can be used to measure the concentration of a target analyte similarly to HPLC-UV.

mentioned alongside HPLC-UV in 0% (65) of relevant job posts

HPLC-UV Complementary Technologies

HPLC-MS

HPLC-MS combines HPLC separation with mass spectrometry detection, providing more detailed structural information than UV detection alone, thus complementing HPLC-UV.

mentioned alongside HPLC-UV in 8% (73) of relevant job posts

LC-MS/MS

LC-MS/MS, like HPLC-MS, is complementary. It offers enhanced sensitivity and specificity through tandem mass spectrometry compared to HPLC-UV.

mentioned alongside HPLC-UV in 2% (108) of relevant job posts

LC-MS

LC-MS combines liquid chromatography with mass spectrometry, providing more detailed structural information than UV detection alone, thus complementing HPLC-UV.

mentioned alongside HPLC-UV in 1% (88) of relevant job posts