What is Hi-C?

Hi-C is a molecular biology technique used to study the three-dimensional organization of chromosomes in the nucleus. It works by cross-linking DNA in cells, fragmenting the DNA, ligating the fragments together, and then sequencing the resulting DNA. The frequency of ligation events provides information about the proximity of different genomic regions in the nucleus. It is commonly used to study chromatin structure, identify chromatin loops, and understand the relationship between genome organization and gene expression.