Gibson assembly
What is Gibson assembly?
Gibson Assembly is a DNA assembly method that allows for the joining of multiple DNA fragments in a single, isothermal reaction. It relies on 5' exonuclease activity to create single-stranded DNA overhangs, followed by annealing of complementary sequences and DNA polymerase to fill in any gaps. DNA ligase then seals the nicks. It's commonly used for cloning multiple DNA fragments into a vector in a specific order and orientation, facilitating the construction of complex genetic circuits, pathways, and synthetic genes.
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