Golden Gate Assembly
What is Golden Gate Assembly?
Golden Gate Assembly is a molecular biology cloning method that allows for the directional assembly of multiple DNA fragments into a single vector in a defined order. It relies on the use of Type IIS restriction enzymes that cut DNA outside of their recognition sequence, allowing for the creation of customized overhangs. These overhangs determine the order in which the DNA fragments are assembled. It is commonly used in synthetic biology and metabolic engineering for constructing multi-gene pathways and complex genetic circuits.
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