Golden Gate cloning
What is Golden Gate cloning?
Golden Gate cloning is a molecular biology technique used to assemble multiple DNA fragments into a single vector in a defined order. It relies on the use of Type IIS restriction enzymes that cleave DNA outside of their recognition sequence. This allows for the design of custom overhangs that dictate the order in which DNA fragments are assembled. The process is typically carried out in a single reaction and is highly efficient, allowing for the construction of complex genetic constructs.
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