dPCR Insights | Sumble

What is dPCR?

Digital PCR (dPCR) is a method for directly quantifying nucleic acids. Unlike real-time PCR (qPCR), dPCR partitions a sample into many individual, separate reactions; some reactions contain the target molecule (positive), and some do not (negative). Following PCR amplification, the fraction of negative reactions is used to determine the absolute number of target molecules in the sample, without the need for standards or endogenous controls. dPCR is commonly used for applications such as rare sequence detection, copy number variation analysis, and accurate gene expression quantification.

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What other technologies are related to dPCR?

dPCR Competitor Technologies

qPCR

qPCR is a method for quantifying DNA amplification in real-time, and can be used for similar applications as dPCR, such as gene expression analysis and pathogen detection, making it a competitor.

mentioned alongside dPCR in 3% (705) of relevant job posts

ELISA

ELISA (Enzyme-Linked Immunosorbent Assay) is a plate-based assay technique for detecting and quantifying substances such as peptides, proteins, antibodies, and hormones. It is a competitor since it is used for quantification like dPCR.

mentioned alongside dPCR in 1% (312) of relevant job posts

NGS

NGS (Next-Generation Sequencing) technologies are competitors as they are used for quantifying nucleic acids.

mentioned alongside dPCR in 1% (195) of relevant job posts

flow cytometry

Flow cytometry is a technique used to count and analyze cells, and it competes with dPCR in certain applications like cell counting and analysis of rare events.

mentioned alongside dPCR in 1% (206) of relevant job posts

next-generation sequencing

Next-generation sequencing technologies are competitors as they are used for quantifying nucleic acids.

mentioned alongside dPCR in 1% (96) of relevant job posts

RT-PCR

RT-PCR is a variant of PCR used for quantifying RNA. Since dPCR can quantify RNA with reverse transcription, RT-PCR is a competitor.

mentioned alongside dPCR in 1% (51) of relevant job posts

Western blot

Western blot is a technique for detecting and quantifying proteins. It is a competitor since it is used for quantification like dPCR, but for proteins.

mentioned alongside dPCR in 0% (53) of relevant job posts