What is scRNAseq?

Single-cell RNA sequencing (scRNA-seq) is a high-throughput technology used to measure the gene expression levels of individual cells. It is commonly used to study cellular heterogeneity, identify rare cell types, and understand developmental processes and disease mechanisms. It involves isolating single cells, lysing them, reverse transcribing RNA into cDNA, amplifying the cDNA, and then sequencing it using next-generation sequencing technologies. The resulting data is then analyzed to determine the expression profile of each cell.