What is quantitative RT-PCR?

Quantitative RT-PCR (qRT-PCR) is a laboratory technique combining reverse transcription of RNA into DNA and amplification of specific DNA targets using polymerase chain reaction (PCR). It is used to quantify the abundance of specific RNA molecules in a sample. The quantification is done in real time, monitoring the amplification process as it occurs, allowing researchers to determine the initial amount of the RNA target. qRT-PCR is commonly used to measure gene expression levels, detect viral RNA, and for diagnostic purposes.