Nucleic acid purification refers to a set of processes used to isolate and purify DNA or RNA from a variety of sources, such as cells, tissues, or biological fluids. The goal is to obtain nucleic acids free from contaminants like proteins, lipids, and other cellular components. Common methods include solid-phase extraction (using silica columns or magnetic beads), liquid-liquid extraction (e.g., phenol-chloroform), and precipitation (e.g., ethanol precipitation). Purified nucleic acids are essential for a wide range of downstream applications in molecular biology, including PCR, sequencing, cloning, and gene expression analysis.
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