HPLC/UPLC Insights

What is HPLC/UPLC?

High-Performance Liquid Chromatography (HPLC) and Ultra-Performance Liquid Chromatography (UPLC) are analytical techniques used to separate, identify, and quantify components in a mixture. HPLC uses high pressure to force the mobile phase through a column packed with a stationary phase. UPLC is a more advanced form of HPLC that uses even higher pressures and smaller particle sizes in the stationary phase to achieve greater resolution, sensitivity, and speed. They are commonly used in pharmaceuticals, environmental monitoring, food chemistry, and clinical research.

Find organizations using HPLC/UPLC on Sumble →

What other technologies are related to HPLC/UPLC?

HPLC/UPLC Competitor Technologies

SFC

Supercritical Fluid Chromatography is an alternative separation technique, thus a competitor.

mentioned alongside HPLC/UPLC in 12% (207) of relevant job posts

LC

Liquid Chromatography (LC) is a broader category that HPLC/UPLC falls under, making it a competitor at that higher level, although closely related. In general use HPLC/UPLC would be considered LC.

mentioned alongside HPLC/UPLC in 7% (246) of relevant job posts

icIEF

icIEF (Imaged Capillary Isoelectric Focusing) is a separation technique based on charge, competing with HPLC.

mentioned alongside HPLC/UPLC in 9% (98) of relevant job posts

CE-SDS

CE-SDS is a capillary electrophoresis technique used for protein separation based on size, thus a competitor.

mentioned alongside HPLC/UPLC in 7% (117) of relevant job posts

capillary electrophoresis

Capillary Electrophoresis is a separation technique using electric fields, thus a competitor.

mentioned alongside HPLC/UPLC in 3% (84) of relevant job posts

GC

Gas Chromatography separates compounds based on their boiling points, thus a competitor.

mentioned alongside HPLC/UPLC in 1% (285) of relevant job posts

SEC

Size Exclusion Chromatography is a type of liquid chromatography which separates molecules by size, so a competitor in terms of the general separation technique but not specifically related to reversed phase separations commonly used with HPLC/UPLC.

mentioned alongside HPLC/UPLC in 2% (73) of relevant job posts

SDS-PAGE

SDS-PAGE (Sodium Dodecyl-Sulfate Polyacrylamide Gel Electrophoresis) separates proteins based on size and charge, so it is a competitor.

mentioned alongside HPLC/UPLC in 1% (86) of relevant job posts

HPLC/UPLC Complementary Technologies

LC-MS

LC-MS combines liquid chromatography with mass spectrometry for enhanced identification and quantification, making it a powerful complementary technique.

mentioned alongside HPLC/UPLC in 3% (381) of relevant job posts

UV-Vis

UV-Vis spectroscopy is a detection method often coupled with HPLC/UPLC, making it complementary.

mentioned alongside HPLC/UPLC in 1% (132) of relevant job posts

UV

UV detection is a common detection method coupled with HPLC/UPLC, making it complementary.

mentioned alongside HPLC/UPLC in 1% (100) of relevant job posts