High-Performance Liquid Chromatography (HPLC) and Ultra-Performance Liquid Chromatography (UPLC) are analytical techniques used to separate, identify, and quantify components in a mixture. HPLC uses high pressure to force the mobile phase through a column packed with a stationary phase. UPLC is a more advanced form of HPLC that uses even higher pressures and smaller particle sizes in the stationary phase to achieve greater resolution, sensitivity, and speed. They are commonly used in pharmaceuticals, environmental monitoring, food chemistry, and clinical research.
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