FRET Insights | Sumble

What is FRET?

Förster resonance energy transfer (FRET) is a mechanism describing energy transfer between two light-sensitive molecules (chromophores). A donor chromophore, initially in its electronic excited state, may transfer energy to an acceptor chromophore through nonradiative dipole-dipole coupling. The efficiency of this energy transfer is highly distance-dependent, making FRET a valuable tool for studying molecular interactions and distances within biological systems. It's commonly used to measure distances between proteins, detect protein conformational changes, and study other dynamic processes in cells.

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What other technologies are related to FRET?

FRET Competitor Technologies

FP

Fluorescence Polarization (FP) is another fluorescence-based technique that measures the polarization of emitted light, often used for similar binding assays as FRET, making it a competitor.

mentioned alongside FRET in 9% (53) of relevant job posts

SPR

Surface Plasmon Resonance (SPR) is a label-free technique that measures binding events by detecting changes in refractive index, offering an alternative to FRET for studying molecular interactions, hence it is a competitor.

mentioned alongside FRET in 3% (75) of relevant job posts

ELISA

ELISA (Enzyme-Linked Immunosorbent Assay) is a plate-based assay technique designed for detecting and quantifying substances such as peptides, proteins, antibodies, and hormones. It can be used for similar quantification purposes as FRET, especially in cases where protein interactions are involved, thus a competitor.

mentioned alongside FRET in 0% (150) of relevant job posts

FRET Complementary Technologies

Western blot

Western blotting is used to detect specific proteins in a sample, and it can be used to confirm the expression and size of proteins that are being studied using FRET, thus it is complementary.

mentioned alongside FRET in 1% (55) of relevant job posts

flow cytometry

Flow cytometry is a technique for counting and examining cells by suspending them in a stream of fluid and passing them by an electronic detection apparatus. FRET can be implemented in flow cytometry to study interactions within or between cells, making flow cytometry a complementary technique.

mentioned alongside FRET in 0% (78) of relevant job posts