What is FRET?

Förster resonance energy transfer (FRET) is a mechanism describing energy transfer between two light-sensitive molecules (chromophores). A donor chromophore, initially in its electronic excited state, may transfer energy to an acceptor chromophore through nonradiative dipole-dipole coupling. The efficiency of this energy transfer is highly distance-dependent, making FRET a valuable tool for studying molecular interactions and distances within biological systems. It's commonly used to measure distances between proteins, detect protein conformational changes, and study other dynamic processes in cells.