droplet digital PCR Insights

What is droplet digital PCR?

Droplet Digital PCR (ddPCR) is a method for performing digital PCR where a sample is partitioned into thousands of nanoliter-sized droplets. Each droplet contains either zero or one (or more) copies of the target molecule. PCR amplification occurs within each droplet, and after PCR, each droplet is read as either positive or negative for the target sequence. This allows for absolute quantification of the target DNA or RNA without the need for external standards. It is commonly used for applications such as rare mutation detection, copy number variation analysis, and gene expression quantification.

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What other technologies are related to droplet digital PCR?

droplet digital PCR Competitor Technologies

qPCR

qPCR (quantitative PCR) is a direct competitor to droplet digital PCR. Both are used for quantifying nucleic acids, but qPCR relies on relative quantification using fluorescent dyes or probes while ddPCR provides absolute quantification.

mentioned alongside droplet digital PCR in 1% (151) of relevant job posts

real-time PCR

Real-time PCR is a synonym for qPCR and as such, a direct competitor to droplet digital PCR for nucleic acid quantification.

mentioned alongside droplet digital PCR in 2% (66) of relevant job posts

droplet digital PCR Complementary Technologies

next-generation sequencing

Next-generation sequencing (NGS) provides comprehensive sequence information, whereas ddPCR offers precise quantification of specific targets. ddPCR can be used to validate NGS results or to quantify specific sequences identified by NGS.

mentioned alongside droplet digital PCR in 1% (76) of relevant job posts