What is ddPCR?

Droplet Digital PCR (ddPCR) is a method for performing digital PCR that partitions a sample into thousands of nanoliter-sized droplets. Each droplet contains either zero or one (or more) copies of the target molecule. PCR is then performed within each droplet individually. After PCR, each droplet is read to determine whether or not the target sequence was amplified. This allows for absolute quantification of the target DNA or RNA without the need for external standards. ddPCR is commonly used for applications such as rare mutation detection, copy number variation analysis, and gene expression quantification.