Column chromatography is a separation technique where the stationary phase is packed in a vertical column. The mixture to be separated is applied to the top of the column, and a solvent (the mobile phase) is passed through the column. Different components of the mixture travel through the column at different rates due to their varying affinities for the stationary and mobile phases, leading to their separation. It is commonly used to purify chemical compounds, separate mixtures, and isolate components from biological samples.
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