Biolayer interferometry (BLI) is an optical label-free technology used to measure biomolecular interactions in real time. It analyzes the interference pattern of white light reflected from two surfaces: a biosensor tip and an internal reference layer. Changes in the number of molecules bound to the biosensor tip cause shifts in the interference pattern, which are measured and used to determine binding kinetics, affinity, and concentration. BLI is commonly used in drug discovery, antibody characterization, and protein-protein interaction studies.
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