What is ATACseq?

ATAC-seq (Assay for Transposase-Accessible Chromatin using sequencing) is a technique used in molecular biology to assess genome-wide chromatin accessibility. It utilizes a hyperactive Tn5 transposase to insert sequencing adapters into open chromatin regions, followed by next-generation sequencing to identify these regions. This allows researchers to map areas of the genome that are accessible to regulatory proteins, thereby providing insights into gene regulation, transcription factor binding, and other chromatin-related processes.