A thermocycler (also known as a thermal cycler, PCR machine or DNA amplifier) is a laboratory apparatus used to amplify segments of DNA via the polymerase chain reaction (PCR). The instrument raises and lowers the temperature of the block in discrete, pre-programmed steps, allowing for denaturation, annealing, and extension of the DNA. Thermocyclers are widely used in molecular biology, genetics, and diagnostic laboratories for a variety of applications, including DNA cloning, sequencing, gene expression analysis, and disease detection.
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